446 IL32β supports the HLA I expression on tumor skin T lymphocytes as a tumor immune escape mechanism in cutaneous T-cell lymphoma

Cutaneous T-cell lymphoma (CTCL), a heterogeneous group of extranodal non-Hodgkin’s lymphoma. In this study, we sequenced skin T cells on single cell level from patients with mycosis fungoides (MF), the most common type CTCL, and segregated clonal malignant non-clonal bystander by natural clonality identifier – receptor (TCR). Based our attention-ANN, an artificial intelligent method, identified 15 cytokine-related gene for identifying cells. By analyzing expression those genes, found that populations show significant increased IL32 decreased IL7R. addition, IL32β as predominant isoform expressed in populations. Furthermore, also secrete into tumor micro-environment, demonstrated elevated protein sera CTCL blood disease, indicating is important autocrine tumorous survival expansion. Interestingly, HLA class I molecules, inhibitory ligand NK cells, positively correlated populations, but not or healthy To prove relevance findings, knocked down siRNA electroporation My-La CD4+ resulting decrease only IL32, three molecules (HLA-A, HLA-B HLA-C). summary, study shows highly isoform, which serves supporting micro-environment. Additionally, correlates extremely helps escape NK-cell immune surveillance.